1. Chemical monitoring method: 3 to 5 chemical indicators that can indicate both temperature and temperature duration are placed in the items to be sterilized, and placed in the sterilizer is the most difficult to achieve sterilization. Part. After a sterilization cycle, the chemical indicator is taken out, and it is judged whether the sterilization condition is reached according to the change in color and properties. 2, physical detection method (thermocouple detection method) Detection method: detection time, the multiple probes of the multi-point temperature detector are placed in each layer of the sterilizer, at the middle and outside points. Close the door and take the wire out. Observe the temperature rise and duration from the recorder. If the temperature (curve) shown reaches the preset temperature, the sterilization temperature is acceptable. 3. Biological detection method (1) Indicating strain: Bacillus subtilis var. niger spore (ATCC9372), and the content of the plaque is 5.0×105 cfu/piece to 5×106 cfu/tablet. The resistance should meet the following conditions: when the temperature is 160 °C ± 2 °C, the D value is 1.3~1.9min, the survival time is ≥3.9min, and the killing time is ≤19min. (2) Detection method: Bacillus subtilis spores were separately placed in a sterile test tube (1 piece/tube). Within the diagonal of the sterilizer and each door handle, two test tubes containing the bacteria are placed at the outer corners, the test tube cap is placed next to the test tube, and the door is closed. After a sterilization cycle, the temperature is lowered to 80 ° C. Remove the test tube cap and remove the test tube. Under sterile conditions, ordinary nutrient broth medium (5 ml/tube) was added and cultured at 36 ° C ± 1 ° C for 48 h. The preliminary results were observed, and the sterile growth tube was further cultured until the seventh day. (3) Judgment of results: If the broth tube inoculated with each indicator piece is clarified, it is judged to be sterilized. If it is pointed out that the broth tube inoculated by one of the pieces is turbid, it is judged as unqualified, and it is difficult to determine the broth. Tube, take 0.1ml inoculated on nutrient agar plate, spread with sterile L rod, and incubated at 36 °C ± 1 °C for 48h, observe the colony morphology, and do smear staining microscopic examination to determine whether there is indicator growth, if there is an indication The growth of the bacteria was judged as unqualified; if there was no growth of the indicator bacteria, it was judged to be sterilized.